Immunoglobulin production by coeliac biopsies in organ culture.
نویسندگان
چکیده
منابع مشابه
Organ culture of mucosal biopsies of human small intestine.
In vitro experiments of small intestinal mucosal function and metabolism utilizing excised tissue have been limited to a few hours by rapid epithelial cell necrosis which occurs with current incubation methods. We describe a method for culturing human mucosal biopsies for up to 24 hr employing organ culture methodology and demonstrate its potential application to studies of mucosal function. Pe...
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The nutritional requirements generally satisfied by serum in established cultures of human hematopoietic cells were studied in spinner culture. The optimum concentration of fetal calf serum for cell proliferation in medium RPMI #1640 appeared to be 20%, and the minimum to be 2%. In the logarithmic phase of cell growth, there was an exponential increase of protein in the cells and a correspondin...
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In vitro cytotoxicity of four different gluten fractions was tested in organ culture for up to 48 hours using flat intestinal biopsies from children with coeliac disease. The fractions were (1) a peptic-tryptic digest of gliadin containing a moderate amount of alpha-gliadin, (2) a peptic-tryptic digest of gluten (Frazer fraction III) froma strain of wheat with a high content of alpha-gliadin, (...
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The histological changes in 95 jejunal biopsy specimens from children have been analyzed by a new mporphometric technique. The microscope image of the specimen is traced directly onto computer data cards. A simple sketch records accurate quantitative data in a matrix of 840 points, retaining the spatial arrangement of the tissue components. The data are fed via an optical mark data card reader,...
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ژورنال
عنوان ژورنال: Gut
سال: 1986
ISSN: 0017-5749
DOI: 10.1136/gut.27.10.1151